The solvent transmission limits (λpr) with the thickness of the absorbing layer (cell) l = 1 cm are:
solvent water chloroform toluene ethanol acetone
λpr, nm 185 245 280 210 320
Which of these solvents cannot be used in the analysis of 2-aminopyrimidine, in the spectrum of which there is an absorption band with max = 225 nm, and why? The transmission value of an aqueous solution of 2-aminopyrimidine with a concentration of 0.010 mg/ml, measured at λ = 225 nm in a cuvette with l = 10 mm, is 21.6%. Calculate the mass, molar and specific absorption coefficients of 2-aminopyrimidine (M = 95 g/mol) at a given wavelength.

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