433. Determine the Michaelis constant and the reversal number of the enzyme α-chymotrypsin in the reaction of hydrolysis of N-acetyl-L-valine methyl ester according to the following data obtained at an initial enzyme concentration of 38 µmol/l at 25 °C in a solution of 0.1 mol/l KCl ([ S]0 is the initial concentration of the substrate, v0 is the initial rate of hydrolysis):
[S]o, mol/L 0.200 0.124 0.091 0.071 0.060
v0*106, mol/(l-s) 4.57 3.83 3.31 2.93 2.67

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